Avian Polyoma virus (Budgerigar Fledgling Disease) PCR

Polyoma virus in birds can be seen clinically as sudden death, neurological signs, abdominal distension (hepatomegaly or ascites), petechial to widespread haemorrhage or failure of development of normal feathers. All psittacine birds can be infected however fledgling and juvenile birds are most susceptible. Chronic, subclinical infection with intermittent viral shedding is common in birds that recover from acute infection. Infection is most commonly due to contact with another infected bird – the virus is shed in feather dander and faeces. The virus can remain stable in the environment for long periods.




Blood, blood spots or feathers


Plain yellow-top container

Collection protocol:

Blood feather - Pluck two blood feathers ensuring there is blood present within the feather quill. Select feathers that are grossly abnormal if PBFDV is supected.

Blood spots - Place a drop of blood on to filter or blotting paper and allow to dry. Blood spots must be at least 6 mm diameter.