Factor VIII deficiency may be suspected in young dogs with a history of haemorrhage and in which other causes have been ruled out, and which have an elevated activated partial thromboplastin time (APTT) with normal prothrombin time (PT).
Collect blood with a minimum of trauma so the coagulation cascade is not triggered, preferably from the jugular so a good flow is ensured. Fill the tube exactly to the level indicated on the blood tube.
Special handling/shipping requirements:
Deliver to the laboratory as soon as possible. If delivery will be delayed, spin to separate the plasma, and aspirate the plasma into a plastic or additive-free tube. Freeze the plasma, and deliver with ice bricks.